Figure 5
From: Characterization of sample preparation methods of NIH/3T3 fibroblasts for ToF-SIMS analysis
Depth profiles were acquired from cells that were freeze-dried (FD, blue bars), formaldehyde-fixed (FF, red bars) and frozen-hydrated (FH, green bars). The average, normalized intensity of the OH-, CN-, Cl-. CNO-, PO2 -, and PO3 - ions were summed over the entire depth profile and plotted. Intensity values for each ion were normalized to the total ion dose. Error bars signify one standard deviation. All depth profiles were acquired for 1000 seconds, using a 500 μm × 500 μm analysis area and a 700 μm × 700 μm sputter area. For the FD cells, n = 10, from four total samples and three separate data acquisition sessions. For the FF cells, n = 7, from three total samples and three separate data acquisition sessions. For the FH cells, n = 11, from three total samples and three separate data acquisition sessions.