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Biointerphases

Journal for Biophysical Chemistry

Table 2 Summary of XPS determined elemental composition (atom %) for NH 2 -SAMs and COOH-SAMs before and after CytoC adsorption

From: Probing the orientation of electrostatically immobilized cytochrome C by time of flight secondary ion mass spectrometry and sum frequency generation spectroscopy

 

At %

     

Sample

Au 4f

C 1s

O 1s

S 2p

N 1s

Normalized N 1s

Bare NH2-SAMs

36.9 (1.5)

53.6 (1.8)

4.6 (0.8)

1.6 (0.3)

3.3 (0.5)

-

Bare COOH-SAMs

43.7 (2.5)

45.9 (2.4)

9.0 (0.3)

1.8 (0.2)

-

-

CytoC on NH2-SAMs (100 μM)

25.2 (0.6)

57.7 (0.9)

8.8 (0.3)

1.1 (0.1)

7.2 (0.4)

6.6 (0.6)

CytoC on COOH-SAMs (100 μM)

25.3 (0.5)

55.5 (1.0)

10.5 (0.5)

1.0 (0.1)

7.6 (0.3)

-

  1. Average experimental compositions were determined from two distinct samples (three spots per sample). All data were collected at a 0º TOA in the hybrid mode. Standard deviations are shown in parentheses.
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