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Biointerphases

Journal for Biophysical Chemistry

Figure 4 | Biointerphases

Figure 4

From: Assessment of cytotoxicity of (N-isopropyl acrylamide) and Poly(N-isopropyl acrylamide)-coated surfaces

Figure 4

Direct contact test results. MTS assay results (first column) for all four cell types after cell culture for 48 and 96 hours on (a) ppNIPAM surfaces, (b) spNIPAM surfaces, (c) frpNIPAM surfaces, and (d) cpNIPAM surfaces. Light microscopy (second column) and LIVE/DEAD assay results (third column) for (a) SMCs after 96 hours of culture on ppNIPAM surfaces, (b) 3T3s after 96 hours of culture on spNIPAM surfaces, (c) BAECs after 96 hours of culture on frpNIPAM surfaces, and (d) Veros after 96 hours of culture on cpNIPAM surfaces. Red line indicates the viability of 70%, below which a compound is considered to be cytotoxic. Asterisks point to the exposed surfaces from which cells have detached (in black). The arrow points to a sheet of detached, live cells.

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