Figure 1

Representative images of A10 vSMCs labeled with Texas Red maleimide after incubation for 24 h on (a) untreated collagen fibril matrices, (b) collagen fibril matrices that have been dehydrated for 24 h, and (c) collagen fibril matrices that have experienced high vacuum (HV, 10 ^-7  Pa to 10 ^-8  Pa) for approximately 6 h. (d) Histogram of cell areas and (e) mean cell areas of A10 vSMC incubated on the different collagen fibril thin matrix states. Image scale bars are 200 μm. Error bars represent the standard deviation of at least three replicate experiments. All samples were made in 50 mm bacterial-grade polystyrene dishes as previously described [34, 37].