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Biointerphases

Journal for Biophysical Chemistry

Table 1 Percent differences between peptide concentrations determined by BCA assay and their nominal concentrations based on dry weight a

From: Evaluating protocols and analytical methods for peptide adsorption experiments

Amino acid

GG–X–GG

X5

X10

 

% Diff. WCb

% Diff. WCb

% Diff. WCb

Glycine (G)

–

17 ± 2

28 ± 9

Alanine (A)

20 ± 2

94 ± 34

–

Aspartic Acid (D)

20 ± 5

157 ± 36

169 ± 24

Glutamic Acid (E)

6 ± 3

128 ± 22

178 ± 5

Lysine (K)

13 ± 6

87 ± 26

101 ± 31

Proline (P)

167 ± 34

193 ± 6

–

Arginine (R)

21 ± 9

105 ± 43

111 ± 41

Serine (S)

26 ± 16

66 ± 25

5 ± 5

  1. aPercent difference defined as absolute difference between BCA- and weight-derived concentrations divided by the mean of the two methods.
  2. bPercent difference values reported as mean ± 95% C.I., n = 3.
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