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Journal for Biophysical Chemistry

Table 2 Comparison of different methods for measuring the solution concentration in 10 mM sodium phosphate buffer (pH 7) of peptides containing chromophoric residues

From: Evaluating protocols and analytical methods for peptide adsorption experiments

Peptide

Weighed conc.

UV abs. (280 nm)

AA Analysis (mean ± 95% C.I., n = 3)

 

(mg/mL)

(mM)

(mM)

% Diff. WCa

(mM)

% Diff. WCa

% Diff. UVb

GGFGG

Low Solubility

–

–

–

–

–

GGWGG

0.41

0.86

0.73

16

–

–

–

GGYGG

0.69

1.53

1.36

12

1.31 ± 0.07

16 ± 6

4 ± 3

GGGGGY

Low Solubility

–

–

–

–

–

GGAGGY

0.55

1.05

0.98

7

0.96 ± 0.04

9 ± 4

2 ± 1

A5Y

Low Solubility

–

–

–

–

–

GGPGGY

0.56

1.01

1.11

9

1.04 ± 0.04

2 ± 3

7 ± 3

P5Y

0.80

1.13

1.27

12

0.97 ± 0.11

15 ± 10

27 ± 9

  1. aW.r.t. the concentration determined by weight.
  2. bW.r.t. the concentration determined by UV absorbance.