Figure 2
From: Cell membrane topology analysis by RICM enables marker-free adhesion strength quantification
Microfluidic cell detachment assay and flow chamber. (A) The detachment assay determines the fraction of adherent cells after exposure to increasing shear stress. PatuT and PatuS cells were allowed to adhere for 90Â min before starting up the flow. Error bars represent the SEM. The critical shear stress Ï„50 where 50% of the cells were detached defines the adhesion strength. A schematic sketch (B) and a photograph (C) of the flow chamber are shown. The flow cell is mounted on a collagen-coated coverslip. The flow chamber consists of two inlets and one outlet. The cells within the flow cell are fluorescently labeled.