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Journal for Biophysical Chemistry

Table 3 HAP content of osteoporotic and non-osteoporotic trabeculae evaluated by XPS and ToF-SIMS

From: Quantification of calcium content in bone by using ToF-SIMS–a first approach

 

12 months sham

12 months diet

XPS analysis

at%

wt%

at%

wt%

C

43.1

30.0

56.2

40.9

O

37.7

35.0

26.3

25.5

N

4.1

3.3

11.1

9.4

Ca

8.6

20.0

3.8

9.2

P

6.5

11.7

2.6

4.9

Calculated HAP (wt%)

 

51.3

 

23.2

Corrected HAP (wt%) XPS

 

59.2

 

26.8

HAP (wt%) ToF-SIMS

51.6

24.0

By calibration curve

  1. HAP content of osteoporotic (12 months diet) and non-osteoporotic (12 months sham) trabeculae of the rat. The evaluation of the HAP content by XPS and ToF-SIMS measurement is described in the Methods section. The error for the quantification by XPS amounts to 10% while for ToF-SIMS the error accounts to 6%, given by the standard error of the slope of Figure 3c (equation (1)).