Figure 2
From: Polarizing cytoskeletal tension to induce leader cell formation during collective cell migration
High levels of curvature increase the motility during migration. Migrating cell collective with spike protrusions at T = 0 (A) and at T = 4 hours (C). Scale bars correspond to 100 μm. (B) Displacement of collective’s perimeter over time visualized for single collective from (A). Color code ranges from red (T = 0) to blue (T = 4 hours). (D) Displacement of the cell collectives’ perimeter relative to original position after boundary removal, averaged over 39 individual collectives. Perimeter displacement in an area corresponding to a 10° angular section plotted versus time. Filled squares correspond to positions with predefined protrusions of high local curvature at 40-50° while gray circles correspond to positions without predefined protrusions at 0-10° in the same collective. Empty circles correspond to circular collective without any protrusions. Dashed lines represent a linear fit to the data of the first 90 minutes of the experiment. Error bars represent standard error of the mean.