Figure 6

Phalloidin staining of multicellular actin structure directly after stencil mask removal. At the position of a highly curved protrusion (A) and at a normal curved perimeter (B). A multicellular actin structure lining the whole perimeter can be seen in B while at the tip of the highly curved protrusion in A a distinct opening in the actin fibers can be observed as indicated by the arrow. Intensity analysis was done in a stripe of 4 μm along the perimeter as seen in (C, magnification of the area of 45 x 68 μm marked in B). The mean fluorescence intensity levels along the perimeter of the collectives with highly curved protrusions are shown in D. Individual quadrants of the perimeter were registered and averaged. At an angle of 45°, corresponding to the highly curved position, a significant dip in intensity is present (P < 0,0001 compared to the relative intensities at angles ranging from 48° to 90°, as well as 0° to 42°). Error bars denote standard error of the mean, N = 432. Scale bars correspond to 50 μm.