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Biointerphases

Journal for Biophysical Chemistry

Figure 3 | Biointerphases

Figure 3

From: Direct patterning of probe proteins on an antifouling PLL-g-dextran coating for reducing the background signal of fluorescent immunoassays

Figure 3

Background fluorescence intensity after Cy3-labelled GST interaction. Five typical surface chemistry protocols: an epoxide treated glass slide saturated with an ethanolamine solution after anti-GST printing (1), a glass slide treated after anti-GST printing with a PLL-g-PEG solution (2), a glass slide treated after anti-GST printing with a PLL-g-dextran solution (3), a glass slide with anti-GST Abs directly printed on a PECVD deposited PEO layer (4) and a glass slide with anti -GST Abs directly printed on a PLL-g-dextran layer obtained by contact printing using a flat stamp according to the protocol explained in Figure 1 (5). An O2 plasma activated glass slide is used as a reference.

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