Journal for Biophysical Chemistry
Table 2 Adsorption of nucleic acids onto hydrotalcite with various anion exchange capacities a
Bound DNA (%) | |||||
|---|---|---|---|---|---|
[HT] (mg/ml) | 100-150°C | 200-150°C | 300-150°C | 400-150°C | 500-150°C |
Pvu4a | |||||
0.08 | 5.3 ± 1.3% | 8.3 ± 2.2% | 19.2 ± 4.4% | 6.6 ± 8.8% | 12.1 ± 7.0% |
0.4 | 18.7 ± 1.8% | 28.4 ± 2.0% | 44.0 ± 9.6% | 30.1 ± 4.1% | 22.6 ± 3.9% |
2 | 41.0 ± 4.7% | 59.1 ± 8.3% | 78.2 ± 3.8% | 78.3 ± 3.0% | 76.5 ± 6.2% |
10 | 92.7 ± 5.5% | 94.2 ± 4.2% | 95.5 ± 5.5% | 92.9 ± 4.4% | 96.2 ± 8.2% |
Pvu4a + cPvu4a | |||||
0.08 | 17.0 ± 0.5% | 11.4 ± 0.2% | 14.8 ± 0.2% | 11.7 ± 0.1% | 13.3 ± 0.4% |
0.4 | 20.3 ± 0.4% | 25.9 ± 0.4% | 28.5 ± 0.5% | 28.5 ± 0.6% | 25.6 ± 0.6% |
2 | 52.6 ± 2.5% | 59.7 ± 4.7% | 77.7 ± 7.0% | 76.1 ± 3.7% | 78.4 ± 6.6% |
10 | 91.9 ± 6.0% | 93.8 ± 4.0% | 96.9 ± 4.6% | 98.0 ± 4.7% | 98.8 ± 3.5% |
pRS316 | |||||
0.08 | 7.5 ± 0.1% | 8.3 ± 0.1% | 11.4 ± 0.4% | 7.1 ± 0.1% | 3.4 ± 0.1% |
0.4 | 19.3 ± 0.3% | 19.8 ± 0.4% | 42.4 ± 2.2% | 31.8 ± 1.6% | 27.3 ± 0.4% |
2 | 57.6 ± 1.8% | 62.8 ± 3.3% | 86.8 ± 6.3% | 83.9 ± 6.5% | 77.0 ± 7.7% |
10 | 87.0 ± 5.3% | 96.5 ± 9.9% | 99.4 ± 30.4% | 99.8 ± 17.6% | 99.7 ± 22.1% |