Skip to main content
Biointerphases

Journal for Biophysical Chemistry

Figure 3 | Biointerphases

Figure 3

From: Helium beam shadowing for high spatial resolution patterning of antibodies on microstructured diagnostic surfaces

Figure 3

Images of antibody-coated surfaces patterned by helium beam exposure through a 45 μ m-mesh mask. (a) An optical microscope image of patterned monoclonal HyHEL-5 antibodies directly detected with goat anti-mouse antibodies conjugated to alkaline phosphatase and BCIP-NBT substrate. (b) SEM image of patterned biotinylated polyclonal goat anti-rabbit antibodies directly detected using silver staining of streptavidin-gold conjugates. (c) An optical microscope image of BCIP-NBT stained E. coli bacteria captured on patterned polyclonal anti-E.coli antibodies on a gold surface.

Back to article page