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Biointerphases

Journal for Biophysical Chemistry

Figure 5 | Biointerphases

Figure 5

From: Helium beam shadowing for high spatial resolution patterning of antibodies on microstructured diagnostic surfaces

Figure 5

Effects of helium beam modification of antibody and BSA, and BSA passivation: protein patterning by helium-beam exposure through a mask with 70 μ m openings and 110 μ m spacing. (I) Optical microscope images of patterns formed by gold nanoparticle-nucleated silver enhancement on surfaces (a) passivated with BSA after beam exposure and incubated with biotinylated lysozyme followed by streptavidin-gold conjugate, or (b) incubated with PBS (no BSA and no lysozyme) after beam exposure and incubated with streptavidin-gold conjugate, or (c) passivated with BSA (no lysozyme) after beam exposure and incubated with streptavidin-gold conjugate. (II) Optical microscope images of patterns formed by 100 nm gold nanoparticle probes on surfaces (d) passivated with BSA after beam exposure, or (e) incubated with PBS (no BSA) after beam exposure, or (f) no PBS pre-incubation.

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