Figure 6

Evaluation of the resolution of the system by helium-beam exposure of antibody-coated surfaces through a mask containing 300nm diameter openings. (a-b) SEM images of patterns formed by beam exposure of HyHEL-5 antibodies followed by BSA passivation, then treated with biotinylated lysozyme followed by streptavidin-gold conjugate and silver enhancement. (d-e) SEM images of patterns formed by beam exposure of HyHEL-5 antibodies followed by PBS (no BSA), then treated with streptavidin-polyHRP conjugate and silver enhancement. (c) and (f) are images are the corresponding control surfaces.