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Biointerphases

Journal for Biophysical Chemistry

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X-ray spectromicroscopy study of competitive adsorption of protein and peptide onto polystyrene-poly(methyl methacrylate)

Biointerphases volume 3, pages FB27–FB35 (2008)Cite this article

Abstract

A synchrotron-based x-ray photoemission electron microscope (X-PEEM) was used to investigate the coadsorption of a mixture of human albumin serum and SUB-6, a synthetic antimicrobial peptide, to a phase-segregated polystyrene/poly(methyl methacrylate) (PMMA) substrate at varying concentrations and pH. The authors show that X-PEEM could detect the peptide adsorbed from solution at concentrations as low as 5.5×10−9 M and could differentiate the four components via near-edge x-ray absorption fine structure spectromicroscopy. At neutral pH the SUB-6 peptide adsorbed preferentially to PMMA. At a pH of 11.8 where the charge on the peptide was neutralized, there was a more balanced adsorption of both species on the PMMA domains. The authors interpret these observations as indicative of the formation of an electrostatic complex between positive peptide and negative protein at pH of 7.0. This solution complex had an adsorption behavior that depended on the polarity of the substrate domains, and favored adsorption to the electronegative PMMA regions. At a pH of 11.8 the complex formation was suppressed and a more competitive adsorption process was observed.

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Authors and Affiliations

  1. BIMR, TuesduesMcMaster University, L8S 4M1, Hamilton, Ontario, Canada

    Bonnie O. Leung & Adam P. Hitchcock

  2. School of Biomedical Engineering, McMaster University, L8S 4L8, Hamilton, Ontario, Canada

    John L. Brash

  3. Advanced Light Source, Lawrence Berkeley National Laboratory, 94720, Berkeley, California

    Andreas Scholl & Andrew Doran

  4. Institut fur Biochemie, Universitatsklinikum Charite, Humboldt-Universitat, 10117, Berlin, Germany

    Peter Henklein

  5. Department of Microbiology and Immunology, University of British Columbia, V6T 1Z4, Vancouver, British Columbia, Canada

    Joerg Overhage, Kai Hilpert, John D. Hale & Robert E. W. Hancock

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  1. Bonnie O. Leung
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  2. Adam P. Hitchcock
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  4. Andreas Scholl
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Leung, B.O., Hitchcock, A.P., Brash, J.L. et al. X-ray spectromicroscopy study of competitive adsorption of protein and peptide onto polystyrene-poly(methyl methacrylate). Biointerphases 3, FB27–FB35 (2008). https://doi.org/10.1116/1.2956637

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  • DOI: https://doi.org/10.1116/1.2956637