Articles
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Citation: Biointerphases 2007 2:20400167
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Nanomaterials and nanoparticles: Sources and toxicity
This review is presented as a common foundation for scientists interested in nanoparticles, their origin, activity, and biological toxicity. It is written with the goal of rationalizing and informing public he...
Citation: Biointerphases 2007 2:20400166 -
Peptide-presenting two-dimensional protein matrix on supported lipid bilayers: An efficient platform for cell adhesion
Understanding and controlling cell adhesion to biomaterials and synthetic materials are important issues in basic research and applied sciences. Supported lipid bilayers (SLBs) functionalized with cell adhesio...
Citation: Biointerphases 2007 2:20400165 -
Combined microslit electrokinetic measurements and reflectometric interference spectroscopy to study protein adsorption processes
Streaming potential/current measurements for the characterization of charge formation processes at solid/liquid interfaces were combined with reflectometric interference spectroscopy. The simultaneous determin...
Citation: Biointerphases 2007 2:20400159 -
Peptid-tethered bilayer lipid membranes and their interaction with Amyloid ß-peptide
The Amyloid peptide (Aβ), a normal constituent of neuronal and non-neuronal cells, has been shown to be a major component of the extracellular plaque of Alzheimer disease (AD). The interaction of Aβ peptides w...
Citation: Biointerphases 2007 2:20400151 -
Settlement and adhesion of algal cells to hexa(ethylene glycol)-containing self-assembled monolayers with systematically changed wetting properties
Protein resistance of self-assembled monolayers (SAMs) of hexa(ethylene glycols) (EG6) has previously been shown to be dependent on the alkoxyl end-group termination of the SAM, which determines wettability [S. H...
Citation: Biointerphases 2007 2:20400143 -
Selective immobilization of biomolecules onto an activated polymeric adlayer
The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functi...
Citation: Biointerphases 2007 2:20400136 -
Design, synthesis, and degradation studies of new enzymatically erodible Poly(hydroxyethyl methacrylate)/poly(ethylene oxide) hydrogels
This work describes the synthesis and the study of poly(hydroxyethyl methacrylate) PHEMA hydrogels, cross-linked by poly(ethylene oxide)(PEO) chains containing the Gly-Gly-Leu tripeptide. This sequence was sel...
Citation: Biointerphases 2007 2:20400131 -
Creation of a mixed poly(ethylene glycol) tethered-chain surface for preventing the nonspecific adsorption of proteins and peptides
Using a heterotelechelic poly(ethylene glycol) (PEG) possessing a mercapto group at one end and an acetal group at the other end (acetal-PEG-SH), the authors constructed a reactive PEG tethered-chain surface o...
Citation: Biointerphases 2007 2:20400126 -
Direct observation of interaction between proteins and blood-compatible polymer surfaces
The adhesion force between blood-compatible polymer (poly(2-methoxyethyl acrylate: PMEA) and proteins (fibrinogen and bovine serum albumin (BSA)) were measured by atomic force microscopy. The PMEA surface show...
Citation: Biointerphases 2007 2:20400119 -
Structural characterization of an elevated lipid bilayer obtained by stepwise functionalization of a self-assembled alkenyl silane film
This work reports a novel tethered lipid membrane supported on silicon oxide providing an improved model cell membrane. There is an increasing need for robust solid supported fluid model membranes that can be ...
Citation: Biointerphases 2007 2:20300109 -
Water at solid surfaces: A review of selected theoretical aspects and experiments on the subject
This review summarizes select aspects of the research on solid/water interfaces. Despite the considerable amount of work, the way water molecules are organized at the interface is still a source of debate. The...
Citation: Biointerphases 2007 2:20300110 -
Temperature dependence of the short-range repulsion between hydrated phospholipid membranes: A computer simulation study
The temperature dependence of the short-range water-mediated repulsive pressure between supported phospholipid membranes is calculated at two intermembrane separations using the grand canonical Monte Carlo tec...
Citation: Biointerphases 2007 2:20300105 -
Nanoscale eluting coatings based on alginate/chitosan hydrogels
The localized availability of bioactive biomolecules directly at the implant/tissue interface presents a promising strategy for improved wound healing and thus biointegration. Bioactive molecules that cannot b...
Citation: Biointerphases 2007 2:20200095 -
Impact of engineered surface microtopography on biofilm formation of Staphylococcus aureus
The surface of an indwelling medical device can be colonized by human pathogens that can form biofilms and cause infections. In most cases, these biofilms are resistant to antimicrobial therapy and eventually ...
Citation: Biointerphases 2007 2:20200089 -
Effect of ionic strength on PNA-DNA hybridization on surfaces and in solution
Peptide nucleic acids (PNAs) are mimics of oligonucleotides containing a neutral peptidelike backbone and are able to bind complementary DNA targets with high affinity and selectivity. In order to investigate ...
Citation: Biointerphases 2007 2:20200080 -
Microfluidic patterning of alginate hydrogels
In this article the authors present techniques which allow the microfluidic design of alginate microgels with layer composition on a chip. The hydrogel is created by combining two laminar flows of the gel prec...
Citation: Biointerphases 2007 2:20200073 -
Atomic force microscopy and surface plasmon resonance investigation of fibronectin interactions with group B streptococci
The interactions of fibronectin (Fn) with group B streptococci (GBS) were investigated using the atomic force microscope (AFM) and surface plasmon resonance (SPR) biosensing. Submonolayer amounts of Fn were im...
Citation: Biointerphases 2007 2:20200064 -
Imaging large arrays of supported lipid bilayers with a macroscope
Herein, the authors present fluorescence resonance energy transfer (FRET) and two-dimensional protein saturation data acquired from spatially addressed arrays of solid supported lipid bilayers (SLBs). The SLB ...
Citation: Biointerphases 2007 2:20200057 -
Generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures
A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was o...
Citation: Biointerphases 2007 2:20100049 -
Site-specific presentation of single recombinant proteins in defined nanoarrays
The authors describe the deposition of single biomolecules on substrates at defined spacing by pure self-assembly. The substrate is equipped with an array of 8 nm large gold particles which form the template f...
Citation: Biointerphases 2007 2:20100044 -
Characterization of protein resistant, grafted methacrylate polymer layers bearing oligo(ethylene glycol) and phosphorylcholine side chains by neutron reflectometry
Neutron reflectometry was used to investigate the structures of end-tethered protein resistant polymer layers based on poly(oligo(ethylene glycol) methyl ether methacrylate) [poly(OEGMA)] and poly(2-methacrylo...
Citation: Biointerphases 2007 2:20100034 -
Molecular-scale structural and functional characterization of sparsely tethered bilayer lipid membranes
Surface-tethered biomimetic bilayer membranes (tethered bilayer lipid membranes (tBLMs)) were formed on gold surfaces from phospholipids and a synthetic 1-thiahexa(ethylene oxide) lipid, WC14. They were charac...
Citation: Biointerphases 2007 2:20100021 -
Structural dynamics of a colloidal protein-mineral complex bestowing on calcium phosphate a high solubility in biological fluids
The concentration of mineral solutes in mammalian blood is considerably higher than that predicted by their solubility product. The plasma protein fetuin-A inhibits calcium phosphate deposition by forming coll...
Citation: Biointerphases 2007 2:20100016 -
Quantitative interpretation of gold nanoparticle-based bioassays designed for detection of immunocomplex formation
The authors present in this paper how the extended Mie theory can be used to translate not only end-point data but also temporal variations of extinction peak-position changes, δλpeak(t), into absolute mass uptak...
Citation: Biointerphases 2007 2:20100006 -
Live cell adhesion assay with attenuated total reflection infrared spectroscopy
Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection ATR infrared IR spectroscopy. In particular, IR spectra were recorded dynamical...
Citation: Biointerphases 2007 2:20100001 -
Poly(l-lysine)-grafted-poly(ethylene glycol)-based surface-chemical gradients. Preparation, characterization, and first applications
A simple dipping process has been used to prepare PEGylated surface gradients from the polycationic polymer poly(l-lysine), grafted with poly(ethylene glycol) (PLL-g-PEG), on metal oxide substrates, such as TiO2 ...
Citation: Biointerphases 2006 1:10400156 -
Benzamidine-based coatings: Implication of inhibitor structure on the inhibition of coagulation enzymes in solution and in vitro hemocompatibility assessment
Synthetic inhibitors of trypsin-like serine proteases were covalently immobilized to polymeric materials to passivate coagulation enzymes during blood contact. The inhibitory potency of a structurally simple a...
Citation: Biointerphases 2006 1:10400146 -
Creation of a functional heterogeneous vesicle array via DNA controlled surface sorting onto a spotted microarray
Membrane protein microarrays are expected to play a key role in the future of drug screening and discovery. The authors present a method for the creation of functional heterogeneous vesicle arrays via DNA cont...
Citation: Biointerphases 2006 1:10400142 -
Enhancement of poly (ethylene glycol) mucoadsorption by biomimetic end group functionalization
Poly(ethylene glycol) (PEG) is widely used in the pharmaceutical, biotechnology, and medical device industries. Although PEG is a biocompatible polymer that has enjoyed widespread use in drug delivery technolo...
Citation: Biointerphases 2006 1:10400134 -
Phagocytosis of poly (L-lysine)-graft-poly (ethylene glycol) coated microspheres by antigen presenting cells: Impact of grafting ratio and poly (ethylene glycol) chain length on cellular recognition
Microparticulate carrier systems have significant potential for antigen delivery. The authors studied how microspheres coated with the polycationic copolymer poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG)...
Citation: Biointerphases 2006 1:10400123 -
Kinetic and affinity analyses of hybridization reactions between peptide nucleic acid probes and DNA targets using surface plasmon field-enhanced fluorescence spectroscopy
Peptide nucleic acid (PNA), a polyamide DNA mimic, has inspired the development of a variety of hybridization-based methods for the detection, quantification, purification, and characterization of nucleic acid...
Citation: Biointerphases 2006 1:10400113 -
Nanosized particles in bone and dissolution insensitivity of bone mineral
Most of the mineral crystals in bone are platelets of carbonated apatite with thicknesses of a few nanometers embedded in a collagen matrix.We report that spherical to cylindrical shaped nanosized particles ar...
Citation: Biointerphases 2006 1:10300106 -
Dynamics of water at membrane surfaces: Effect of headgroup structure
Atomistic molecular dynamics simulations of fully hydrated 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-phosphatidylethanolamine (POPE), and 1-palmitoyl-2-oleoyl-phosphatidylglycerol (P...
Citation: Biointerphases 2006 1:10300098 -
Modeling of pattern development during fibronectin nanofibril formation
Formation of fibrillar fibronectin networks is a major process during embryogenesis and tissue formation, but the molecular details of fibril assembly remain poorly understood. Based on current ideas of fibron...
Citation: Biointerphases 2006 1:10300093 -
Mixed DNA/oligo (ethylene glycol) functionalized gold surfaces improve DNA hybridization in complex media
Reliable, direct “sample-to-answer” capture of nucleic acid targets from complex media would greatly improve existing capabilities of DNA microarrays and biosensors. This goal has proven elusive for many curre...
Citation: Biointerphases 2006 1:10200082 -
Surface plasmon optical detection of β-lactamase binding to different interfacial matrices combined with fiber optic absorbance spectroscopy for enzymatic activity assays
In this study, we describe the attachment of biotin-functionalized β-lactamase to different types of interfacial architectures. Generic biotin-NeutrAvidin binding matrices were assembled using biotin-terminate...
Citation: Biointerphases 2006 1:10200073 -
Mineralized biological materials: A perspective on interfaces and interphases designed over millions of years
The one feature that distinguishes mineralized biological from synthetic materials is the presence in mineralized biological materials of a complex assemblage of macromolecules. This assemblage usually acts as...
Citation: Biointerphases 2006 1:10200012 -
Directions in peptide interfacial science
The evolution of biological surface science can be credited to the development of traditional surface-chemistry tools and techniques to investigate molecular and atomic-scale bonding, structure, conformation, ...
Citation: Biointerphases 2006 1:10200005 -
Microfabricated three-dimensional environments for single cell studies
Citation: Biointerphases 2006 1:10100064 -
Probing surfaces with single-polymer atomic force microscope experiments
In the past 15 years atomic force microscope (AFM) based force spectroscopy has become a versatile tool to study inter- and intramolecular interactions of single polymer molecules. Irreversible coupling of pol...
Citation: Biointerphases 2006 1:10100063 -
A surface scientist’s perspective on Biointerphases, or “Out of the Vacuum, into the Liquid”
Citation: Biointerphases 2006 1:10100062 -
Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide
Thorough studies of protein interactions with stimulus responsive polymers are necessary to provide a better understanding of their applications in biosensors and biomaterials. In this study, protein behavior ...
Citation: Biointerphases 2006 1:10100061 -
Protein resistant surfaces: Comparison of acrylate graft polymers bearing oligo-ethylene oxide and phosphorylcholine side chains
The objective of this work was to compare poly(ethylene glycol) (PEG) and phosphorylcholine (PC) moieties as surface modifiers with respect to their ability to inhibit protein adsorption. Surfaces were prepare...
Citation: Biointerphases 2006 1:10100050 -
Monitoring kinetics of surface initiated atom transfer radical polymerization by quartz crystal microbalance with dissipation
This article reports that the kinetics of surface-initiated atom transfer radical polymerization can be quantified by the quartz crystal microbalance with dissipation (QCM-D) technique. The kinetics of in situ gr...
Citation: Biointerphases 2006 1:10100035 -
Structural and kinetic properties of laterally stabilized, oligo(ethylene glycol)-containing alkylthiolates on gold: A modular approach
The formation of highly ordered self-assembled monolayers (SAMs) on gold from an unusually long and linear compound HS(CH2)15CONH(CH2CH2O)6CH2CONH(CH2)15CH3 is investigated by contact angle goniometry, ex situ nu...
Citation: Biointerphases 2006 1:10100022 -
Settlement behavior of swimming algal spores on gradient surfaces
When surfaces possessing gradients of surface energy are incubated with motile spores from the green seaweed Ulva, the spores attach on the hydrophilic part of the gradient in larger numbers than they do on the h...
Citation: Biointerphases 2006 1:10100018 -
Breaking bonds in the atomic force microscope: Extracting information
A theoretical framework is developed to analyze molecular bond breaking in dynamic force spectroscopy using atomic force microscopy (AFM). An analytic expression of the observed bond breaking probability as a ...
Citation: Biointerphases 2006 1:10100011 -
Robust hybrid thin films that incorporate lamellar phospholipid bilayer assemblies and transmembrane proteins
This study describes facile methods based on sol-gel processing for the formation of robust thin films that incorporate phospholipid bilayer membranes and transmembrane proteins as multilamellar assemblies in ...
Citation: Biointerphases 2006 1:10100006 -
Spiral twisting of fiber orientation inside bone lamellae
The secondary osteon — a fundamental building block in compact bone — is a multilayered cylindrical structure of mineralized collagen fibrils arranged around a blood vessel. Functionally, the osteon must be ad...
Citation: Biointerphases 2006 1:10100001